The objective of this work was to optimize sugar concentrations, nitrogen and phosphorus in the medium, in order to increase the efficiency production, establishing the influence of these macronutrients in the flavor compounds listed in the Mexican legislation (NOM-006-SCFI-2005). It was used two strains of Saccharomyces yeasts and two types oO must: 100% Agave and mixeO Agave (.Agave plus sucrose). At first it was used a factorial design (2(4-1)). Control variables: sugar concentration, nitrogen and phosphorus, and temperature. Response variables: fermentation efficiency and concentrations of the compounds listed in the NOM. The objective function was determined by minimum square r for each response variable with its restrictions. For the efficiency of 94.58% (with a desirable function of 0.891312), the program suggested, mixed Agave (Agave and sucroee), Saccharomyces THL 110; 8°Bx; 0.797979 g /1 N; 0.376875 g / 1 P, and at 40 °C.

El objetivo del trabajo fue optimizar las concentraciones de azúcar, nitrógeno y fósforo del medio, para incrementar la eficiencia en la producción y establecer la influencia de esos macronutrientes en los compuestos sensoriales indicados en la normatividad Mexicana (NOM -006-SCFI-2005). Se utilizaron dos cepas de levaduras Saccharomyces y dos tipos de mosto: 100% Agave y Mixto (Agave más sacarosa). En un principio se utilizó un diseño factorial (2(4-1)). Variables a controlar: concentración de azúcar; nitrógeno y fósforo; y la temperatura. Variables de respuesta: Eficiencia de fermentación y las concentraciones de los compuestos indicados en la NOM. Por mínimos cuadrados se determinó la función objetivo para cada variable de respuesta con sus restricciones. Para una eficiencia de 94.58% (con una función deseable de 0.891312), el programa sugirio: mosto mixto (Agave y sacarosa), Saccharomyces THL 110; 8°Bx; 0.797979 g/l N; 0.376875 g/l P, y 40 °C.

In this study, the fixed - bed adsorption of carbon dioxide and a carbon dioxide - nitrogen mixture on zeolite 13X was investigated. The adsorption equilibrium and breakthrough curves were determined at different temperatures - 301 - 306 K, 323 K, 373 K and 423 K. A model based on the LDF approximation for the mass transfer, considering the energy and momentum balances, was used to describe the adsorption kinetics of carbon dioxide and a carbon dioxide - nitrogen mixture. The model acceptably reproduced all of the breakthrough curves and can be considered as adequate for designing a PSA cycle to separate carbon dioxidenitrogen mixtures.

Almost identical polyglutamine-containing proteins with unknown structures have been found in human, mouse and rat genomes (GenBank AJ277365, AF525300, AY879229). We infer that an identical new gene (RING) finger domain of real interest is located in each C-terminal segment. A three-dimensional (3-D) model was generated by remote homology modeling and the functional implications are discussed. The model consists of 65 residues from terminal position 707 to 772 of the human protein with a total length of 796 residues. The 3-D model predicts a ubiquitin-protein ligase (E3) as a binding site for ubiquitin-conjugating enzyme (E2). Both enzymes are part of the ubiquitin pathway to label unwanted proteins for subsequent enzymatic degradation. The molecular contact specificities are suggested for both the substrate recognition and the residues at the possible E2-binding surface. The predicted structure, of a ubiquitin-protein ligase (E3, enzyme class number 6.3.2.19, CATH code 3.30.40.10.4) may contribute to explain the process of ubiquitination. The 3-D model supports the idea of a C3HC4-RING finger with a partially new pattern. The putative E2-binding site is formed by a shallow hydrophobic groove on the surface adjacent to the helix and one zinc finger (L722, C739, P740, P741, R744). Solvent-exposed hydrophobic amino acids lie around both zinc fingers (I717, L722, F738, or P765, L766, V767, V733, P734). The 3-D structure was deposited in the protein databank theoretical model repository (2B9G, RCSB Protein Data Bank, NJ).

We present new experimental atomic transition values for the atomic emission spectrum of doubly ionized argon, Ar III. In this work we studied 3s² 3p4 - 3s² 3p³ 3d, 3s² 3p4 - 3s² 3p³ 4s, 3s² 3p4 - 3s² 3p³ 4d, 3s² 3p4 - 3s² 3p³ 5s, 3s² 3p³ 4p, and 3s² 3p³ 4p transition arrays and 196 lines were identified as new atomic transitions between levels of these configurations. The experimental data were obtained from a capillary-discharge tube. The identifications are supported by multiconfiguration Hartree-Fock relativistic calculations.